Hi Careina,
One thing I'd mention in addition to the spot-on replies so far is that
cryoprotection strategies can influence diffraction. These look like pretty
spherulites though, so best of luck in optimization!
Andrew
On Wed, Nov 8, 2023, 10:03 AM careinaedgo...@yahoo.com <
02531c126adf-dma
Along the lines of mass spec analysis, but probably more accessible to
many, is gel electrophoresis. Rinsing enough crystals and dissolving them
in your protein buffer should give you a sample suitable for standard
agarose and polyacrylamide gel electrophoresis protocols. I showed that
crystals I h
Hi Andre,
I recently prepared a Consurf-like figure using conservation scores pulled
from alignments in JalView. You would follow instructions for running
data2bfactor.py in PyMOL, feeding the conservation scores to replace your
model's B factor column. Then set up a color ramp like you would use t
. . . That one time people tested out turning the CCP4BB into an
electronic, dry speakeasy.
This thread confused me for some time, hah!
On Wed, Mar 12, 2025, 10:57 PM Harry Powell <
193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote:
> bingo.
>
> But now everone knows…
>
> Harry
>
> > On 11 Ma
Look into options like lockdown mode on Android.
On Thu, Mar 20, 2025, 7:26 PM Goldman, Adrian
wrote:
> Oh, and on the same topic: if I remember rightly, US law means that you
> don’t have to reveal your mobile phone password - but you can be forced to
> do biometric unlocking (fingerprint, faci
Help us understand a bit more about what you are saying about Gloria
Borgstahl.
On Thu, Mar 20, 2025, 5:12 PM Carter, Charlie <
0001086f1a20d42f-dmarc-requ...@jiscmail.ac.uk> wrote:
> Timothy Snyder says it thus in Rule One from *On Tyranny*:
>
> Do not obey in advance!
>
> Too many, unfortuna
n Thu, Mar 20, 2025, 7:23 PM Andrew Gnann wrote:
> Help us understand a bit more about what you are saying about Gloria
> Borgstahl.
>
>
>
>
> On Thu, Mar 20, 2025, 5:12 PM Carter, Charlie <
> 0001086f1a20d42f-dmarc-requ...@jiscmail.ac.uk> wrote:
>
>> Timo
hah!
On Fri, Mar 21, 2025, 6:44 PM Harry Powell <
193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote:
> Hi Gottfried (and Karl!, and Andrew Gnann off-board)
>
> Many thanks. It seems that if I don’t have the “set_view()” as the last
> thing I do before I save the picture, the
It'd be great if everyone didn't give up on us.
Also, it occurs to me that some commenting here may not have read the US
constitution:
https://www.archives.gov/founding-docs/constitution-transcript
Whereas there is place for speaking from the heart as we have, we should
also prepare ourselves wi
