Since I wrote this message, I found a good reference about radiation damage in
cryoEM. Sharing in case it is helpful to others:
https://doi.org/10.1016/S0076-6879(10)81015-8
Cheers,
Guillaume
From: Guillaume Gaullier
Sent: Tuesday, March 18, 2025 12:03:18 PM
? ?
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
Original Message
On 20/03/2025 10:12, Carter, Charlie wrote:
> Timothy Snyder says it thus in Rule One fromOn Tyranny:
>
> Do not obey in advance!
>
> Too many, unfortunately will decide to
Dear Sir,
I have a query regarding the Biacore 1k+ - NTA chip experiment. I have
performed an interaction study between a his-tagged protein and peptide. I
am facing some problems in the experiment, and I am guessing it is with the
EDTA regeneration.
The response of the 0 µM concentration (1X PBS
Hi Silvia, et al.
Four quick thoughts on what lies below:
1. The French should demand the immediate return of the Statue of Liberty.
2. Everyone should use Signal with end-to-end encryption, and disappearing
messages, for personal communication. (Just because you are paranoid, it does
not mea
Dear All,
This year Instruct-ERIC is providing dedicated funding for technology
development R&D projects within the area of "AI tools for Integrated structural
biology". Scientists at any career stage can apply, and if successful will
receive a maximum of €30,000 of funding for their project.
Dear Kay,
do you use devtoolset (Red Hat Developer Toolset)? It's a set of
relatively new tools and compilers, up to GCC 12, for RHEL7. It might
be the best solution unless you need GCC 13+.
Marcin
On Wed, Mar 26, 2025 at 6:18 PM Kay Diederichs
wrote:
>
> Dear all,
>
> when compiling XDS or any
Dear All,
Rakhi Rajan and I are co-chairing the session "Combining experimental data
with predicted models for structure determination" session at the 75th
American Crystallographic Association Meeting in Lombard, IL (near Chicago)
from July 18–23, 2025. It's a great meeting, and we encourage both
Timothy Snyder says it thus in Rule One from On Tyranny:
Do not obey in advance!
Too many, unfortunately will decide to obey in advance, when a unified
resistance would serve us all better in the long run. We need more to chose the
path cleared by Glorian Borgstahl.
Charlie
On Mar 12, 2025,
Hi all,
Just a quick notice if you are PyVOL users.
I have accidentally updated my PyMOL to version 2.6.1 (I find version 3.1
horrible) and found out that the latest PyVOL 1.7.8 installs correctly but
doesn’t work due to some deprecated arguments in the code.
If you want to keep using it, do not
I think James Holton's "build_pdb" project can do this.
For example, to make a canonical alpha helix,
#!/bin/csh
cat << EOF | build_n2c.awk | tee helix.pdb
BUILD MET 0 -47 180
BUILD ALA -57 -47 180
BUILD ALA -57 -47 180
BUILD ALA -57 -47 180
BUILD ALA -57 -47 180
BUILD ALA -57 -47 180
BUILD ALA
Dear Community,
First of all, apologies for the slightly off-topic question, which is related
to protein purification rather than crystallography.
I am seeking your advice on preparing the cell pellets for the lysis process.
We have an LM20 microfluidizer and we experience quite frequent cloggi
It'd be great if everyone didn't give up on us.
Also, it occurs to me that some commenting here may not have read the US
constitution:
https://www.archives.gov/founding-docs/constitution-transcript
Whereas there is place for speaking from the heart as we have, we should
also prepare ourselves wi
There is a way to do this in pymol. Change the mouse mode to editing mode and
click the two ends of the loop, then you should be able to drag it around. I
don’t remember exactly how, but hopefully this helps.
Alternatively, you could delete the loop and input the structure into modeller
and ask
Dear CCP4BBers/RADDAMers
Some of you may have noticed that the registration for the RD12 Workshop closed
2 weeks ago earlier than advertised, since we had already reached the capacity
of the PSI room we had booked.
We have now been able to move the Workshop to a bigger room, so registration is
n
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