Re: [ccp4bb] sterile filter for protein preps

2025-04-01 Thread Gloria Borgstahl
Unfortunately azide binds to the active site metal of MnSOD, shoot I forgot it was April Fools Day! On Tue, Apr 1, 2025, 3:32 PM Patrick Loll wrote: > Given that it’s April 1st, I’m gonna say you should just autoclave it. > > Or more seriously, back in the day we used to use 0.05% sodium azide,

Re: [ccp4bb] sterile filter for protein preps

2025-04-01 Thread Patrick Loll
Given that it’s April 1st, I’m gonna say you should just autoclave it. Or more seriously, back in the day we used to use 0.05% sodium azide, which kept the bugs at bay pretty well. Obviously this can be problematic for heme proteins, but in general it’s worth considering. Pat ---

Re: [ccp4bb] sterile filter for protein preps

2025-04-01 Thread Jurgen Bosch
Hi Gloria, We use Millipore Millex PVDF 0.22 to filter our mAbs so we can perform growth inhibition assays with our various plasmodium cultures. We do this by syringe in a sterile hood. We keep our sterile filter mAbs at 4C though for about 6 months. We also freeze them and /or lyophilize them for

[ccp4bb] Postdoc at MRC-LMB, Cambridge UK

2025-04-01 Thread Suyang Zhang
Dear all, We are looking for an enthusiastic postdoc to join our team to investigate the mechanism of transcription-coupled splicing. The project aims to understand the crosstalk between the transcription and splicing machineries using a combined biochemical and structural approach with a focus

Re: [ccp4bb] microfluidizer

2025-04-01 Thread Cyprian Cukier
Thank you for all the replies with various suggestions and guidelines. We will adjust our workflows, and let’s hope the clogging will not appear (at least not so often). Best regards Cyprian From: CCP4 bulletin board On Behalf Of Diogo V.M. Melo Sent: Thursday, March 27, 2025 10:46 AM To: CCP4

[ccp4bb] sterile filter for protein preps

2025-04-01 Thread Gloria Borgstahl
Hello Dear Friends in Structural Biology, We need to store a protein at RT and I think it would be best to sterile filter it. Does anyone do this? What filters do you use? Diversely yours! Gloria To unsubscribe from the C