Unfortunately azide binds to the active site metal of MnSOD, shoot I forgot it was April Fools Day!
On Tue, Apr 1, 2025, 3:32 PM Patrick Loll <pjl...@gmail.com> wrote: > Given that it’s April 1st, I’m gonna say you should just autoclave it. > > Or more seriously, back in the day we used to use 0.05% sodium azide, > which kept the bugs at bay pretty well. Obviously this can be problematic > for heme proteins, but in general it’s worth considering. > > Pat > > > --------------------------------------------------------------------------------------- > Patrick J. Loll, Ph. D. (he, him, his) > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St > <https://www.google.com/maps/search/245+N.+15th+St?entry=gmail&source=g>., > Mailstop 497 > Philadelphia, PA 19102 USA > > (215) 762-7706 > pjl...@gmail.com > pj...@drexel.edu > > > On Apr 1, 2025, at 4:21 PM, Gloria Borgstahl <gborgst...@gmail.com> > wrote: > > > > Hello Dear Friends in Structural Biology, We need to store a protein at > RT and I think it would be best to sterile filter it. Does anyone do > this? What filters do you use? > > Diversely yours! Gloria > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > Patrick Loll > pjl...@gmail.com > > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
