Re: [ccp4bb] microfluidizer

Dear Cyprian, I recommend to users to use a Potter-Elvehjem homogenizer before to use the LM20, or even (in case of non fragile protein) to do some cycle of sonicator. I advice also to pay attention to the anti-drop ring of the used bottle. Because in case of old one, you may have some plastic

Re: [ccp4bb] Unexplained electron density

"The protein was neither crystallized nor purified with any substrate" On returning to an old project, I discovered that a site believed to be empty was actually at around 25% occupancy in the crystal structure, with the native ligand. Since the ligand was not added to the crystallization mix,

Re: [ccp4bb] Unexplained electron density

Hi Manjula, Since this is an enzyme, if you are able to share its expected functionality then this community might be able to help better. Best wishes, Partha On Tue, Mar 25, 2025 at 1:08 PM Manjula Ramu wrote: > Dear All, > > I have a trimeric protein structure with unexplained electron densit

[ccp4bb] Open positions in the Structural Biology and Biophysics Unit at Fondazione Ri.MED

Dear all I would like to bring to your attention two open positions in the Structural Biology and Biophysics Unit at Fondazione Ri.MED (*https://www.fondazionerimed.eu/?lang=en *): 1. Postdoctoral Research Fellowship in Structural Biology applied to Drug D

Re: [ccp4bb] microfluidizer

Dear all, A note on high pressure homogeniser designs. Clogging is the main problem with all fixed geometry disruption channel instruments. When selecting a cell disruptor for my laboratory I came across the Stansted high pressure variable geometry chamber homogeniser. The "disruption" gap is