Dear Cyprian, I recommend to users to use a Potter-Elvehjem homogenizer before to use the LM20, or even (in case of non fragile protein) to do some cycle of sonicator.
I advice also to pay attention to the anti-drop ring of the used bottle. Because in case of old one, you may have some plastic particles which falls in the sample and clog the LM20 cell. Of course, dust is not welcome neither… To unclog the cell there is no other choice than to invert it. In case of severe clogging, the water-bath sonicator can help (gentle heating and sonication), before to retry to unclog the cell mounted in a reverse way. Best. Michel. > Le 25 mars 2025 à 07:09, Cyprian Cukier <cyprian.cuk...@selvita.com> a écrit : > > > Dear Community, > > First of all, apologies for the slightly off-topic question, which is related > to protein purification rather than crystallography. > > I am seeking your advice on preparing the cell pellets for the lysis process. > We have an LM20 microfluidizer and we experience quite frequent clogging of > the system that is tedious to remove. This happens particularly with the E. > coli pellets, but from time to time the eukaryotic cells are also > problematic. Does anyone have well-established protocols on how to handle the > cell pellets before applying them to the lysis process in the microfluidizer? > > I should add that of course we follow the manufacturer's recommendations to > have a homogenous suspension, which is not too dense. > > I would also appreciate any advice on how you clean your systems (regularly > or in case of clogging). > > Best regards > Cyprian > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
