Dear all, A note on high pressure homogeniser designs.
Clogging is the main problem with all fixed geometry disruption channel instruments. When selecting a cell disruptor for my laboratory I came across the Stansted high pressure variable geometry chamber homogeniser. The "disruption" gap is formed between the conical surface of the chamber and a needle on an "air suspension". This solution allows particles to push the needle off and temporarily widen the chamber gap, preventing blockages. We have not had a single clogging incident with their SPCH-10 homogeniser after 3 years of use. https://www.homogenisingsystems.com/laboratory_high_pressure_homogenizers.html Oleksiy sent from mobile On 25 Mar 2025 8:44 am, M T <michel...@gmail.com> wrote: Dear Cyprian, I recommend to users to use a Potter-Elvehjem homogenizer before to use the LM20, or even (in case of non fragile protein) to do some cycle of sonicator. I advice also to pay attention to the anti-drop ring of the used bottle. Because in case of old one, you may have some plastic particles which falls in the sample and clog the LM20 cell. Of course, dust is not welcome neither… To unclog the cell there is no other choice than to invert it. In case of severe clogging, the water-bath sonicator can help (gentle heating and sonication), before to retry to unclog the cell mounted in a reverse way. Best. Michel. Le 25 mars 2025 à 07:09, Cyprian Cukier <cyprian.cuk...@selvita.com> a écrit : Dear Community, First of all, apologies for the slightly off-topic question, which is related to protein purification rather than crystallography. I am seeking your advice on preparing the cell pellets for the lysis process. We have an LM20 microfluidizer and we experience quite frequent clogging of the system that is tedious to remove. This happens particularly with the E. coli pellets, but from time to time the eukaryotic cells are also problematic. Does anyone have well-established protocols on how to handle the cell pellets before applying them to the lysis process in the microfluidizer? I should add that of course we follow the manufacturer's recommendations to have a homogenous suspension, which is not too dense. I would also appreciate any advice on how you clean your systems (regularly or in case of clogging). Best regards Cyprian ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
