[ccp4bb] Data curation post for EMDB and EMPIAR

Dear all We have a post opening at EMBL-EBI, Hinxton UK to work on EMDB and EMPIAR data curation at a very exciting time when the archives are expanding rapidly both in volume and richness of content: https://www.embl.de/jobs/searchjobs/index.php?ref=EBI01684&newlang=1&pos%5B%5D=0&loc%5B%5D=2

[ccp4bb] Vacancies: PSI-FELLOW-III-3i / Postdoctoral Fellows at Swiss Light Source, Switzerland

We are looking for two postdoctoral fellows to join macromolecular crystallography (MX) team at the Swiss Light Source for the following projects: ID-Nr. Name of the PI Firstname Theme Email Division 6000-12 Wojdyla Justyna Covid-19 X-ray diffraction data analysis for fragment scre

Re: [ccp4bb] Vacancies: PSI-FELLOW-III-3i / Postdoctoral Fellows at Swiss Light Source, Switzerland

Please, make sure to copy and paste the link into your browser - otherwise you get redirected to the wrong job advert. Apologies about that. https://www.psi.ch/en/pa/job-opportunities/38917-psi-fellow-iii-3i-postdoctoral-fellows-fmd Best regards, Justyna --

[ccp4bb] Vacancy hiring: Postdoctoral Research Assistant in Protein Design and Synthetic Biology at University of Bristol

Research Assistant in Protein Design and Synthetic Biology With Dr Fabio Parmeggiani, University of Bristol The group of Dr Fabio Parmeggiani is seeking a talented and enthusiastic postdoc interested in protein design and synthetic biology. The project will be focused on designing custom modul

Re: [ccp4bb] taking information from a deposited structure

Each PDB entry now has a DOI which should make referencing that structure easier in your own publication. From: CCP4 bulletin board on behalf of Firdous Tarique Reply-To: Firdous Tarique Date: Wednesday, September 9, 2020 at 1:35 AM To: "CCP4BB@JISCMAIL.AC.UK" Subject: Re: [ccp4bb] taking in

[ccp4bb] dimeric tag to induce the homodimerization of protein

Hi I want to make my protein dimeric to increase its affinity for its interaction partner which is a dimer. does anyone know a suitable tag/fusion protein which can be used as C terminal fusion for this purpose? I can not use any of the leucine zipper as I am looking for the distance between

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

Any dimeric tag should work if you add a long enough linker to satisfy your distance criterion. GST, for example. Download the coordinates and get a rough idea how long the linker would have to be for your protein. Diana ** Diana R. Tomchick Prof

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

Hi Dhiraj, You could try a C-terminal Fc-tag if it is an extracellular protein, e.g. see pHL-FcHis vector described in the supplement of PMID 17001101. Best wishes, Tomas On Tue, Sep 22, 2020 at 6:08 PM Srivastava, Dhiraj wrote: > > Hi > I want to make my protein dimeric to increase its aff

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

I was thinking the form of GFP that dimerizes. This would also make it easy to track where the protein is. On Tue, Sep 22, 2020 at 1:28 PM Diana Tomchick < diana.tomch...@utsouthwestern.edu> wrote: > Any dimeric tag should work if you add a long enough linker to satisfy > your distance criterion

[ccp4bb] Student Summer Internships at Merck

CCP4 colleagues- If you know of current undergraduate or PhD students that might be interested in a career in industry, please alert them to Merck summer internship opportunities at any one of our research sites in the greater New York City, Boston, San Francisco, or Philadelphia areas. We are