Hi Dhiraj, You could try a C-terminal Fc-tag if it is an extracellular protein, e.g. see pHL-FcHis vector described in the supplement of PMID 17001101.
Best wishes, Tomas On Tue, Sep 22, 2020 at 6:08 PM Srivastava, Dhiraj <dhiraj-srivast...@uiowa.edu> wrote: > > Hi > I want to make my protein dimeric to increase its affinity for its > interaction partner which is a dimer. does anyone know a suitable tag/fusion > protein which can be used as C terminal fusion for this purpose? I can not > use any of the leucine zipper as I am looking for the distance between the c > terminus to be around 30-40 A. > > > Thank you > Dhiraj > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
