I was thinking the form of GFP that dimerizes. This would also make it easy to track where the protein is.
On Tue, Sep 22, 2020 at 1:28 PM Diana Tomchick < diana.tomch...@utsouthwestern.edu> wrote: > Any dimeric tag should work if you add a long enough linker to satisfy > your distance criterion. > > GST, for example. Download the coordinates and get a rough idea how long > the linker would have to be for your protein. > > Diana > > ************************************************** > Diana R. Tomchick > Professor > Departments of Biophysics and Biochemistry > UT Southwestern Medical Center > 5323 Harry Hines Blvd. > Rm. ND10.214A > Dallas, TX 75390-8816 > diana.tomch...@utsouthwestern.edu > (214) 645-6383 (phone) > (214) 645-6353 (fax) > > On Sep 22, 2020, at 12:08 PM, Srivastava, Dhiraj < > dhiraj-srivast...@uiowa.edu> wrote: > > EXTERNAL MAIL > > Hi > I want to make my protein dimeric to increase its affinity for its > interaction partner which is a dimer. does anyone know a suitable > tag/fusion protein which can be used as C terminal fusion for this purpose? > I can not use any of the leucine zipper as I am looking for the distance > between the c terminus to be around 30-40 A. > > > Thank you > Dhiraj > > ------------------------------ > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > CAUTION: This email originated from outside UTSW. Please be cautious of > links or attachments, and validate the sender's email address before > replying. > > > ------------------------------ > > UT Southwestern > > Medical Center > > The future of medicine, today. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
