[ccp4bb] PostDoc position at EMBL Grenoble to work on ESRF beamline MASSIF-1

Dear All, we have a post-Doc position available here at EMBL Grenoble shared between the high throughput crystallisation team (https://www.embl.fr/services/ht_crystallisation/index.html) and the synchrotron team (https://www.embl.fr/research/unit/mccarthy/index.html) to work with me on ESRF b

Re: [ccp4bb] CCP4MG cannot save images in Mac OS

Dear Jerome, It is entirely possible that this is a Catalina problem. I do not have a Catalina machine and hence have not tested CCP4MG on Catalina. I will install Catalina on one of my computers right away and see if I get the same problem. It may also depend on what graphics chip you have in y

[ccp4bb] PhD positions in Synthetic and Structural Biology in the Max Planck Centre for Minimal Biology, Bristol UK

Dear All, We are looking for highly motivated and ambitious individuals to progress our R&D programme on synthetic viral nanosystems (SVNs), to fill two PhD posts available: one in next-generation vaccines and one in novel genome engineering tools. The projects are in the frame of the BBSRC funde

[ccp4bb] ReNaFoBiS National School OLERON 2020

ReNaFoBiS. Oleron 2020. We are pleased to announce the 7th RéNaFoBiS Oléron workshop, dedicated to Integrative Structural Biology, that will take place on the Oléron island (France), from June the 12th until June the 19th, 2020. The main objective of this workshop is to offer a theoretical an

Re: [ccp4bb] CCP4MG cannot save images in Mac OS

Dear Jerome, On my 13in Mid 2014 MacBook Pro with Intel Iris Graphics now running Catalina (10.15.1), CCP4MG screenshots seem to work fine. What Computer/Graphics chip are you using? I'll have to try upgrading a different computer to see if I can reproduce the problem. Best wishes, Stuart On

Re: [ccp4bb] CCP4MG cannot save images in Mac OS

Dear Jerome, Jon Agirre has pointed to me out you wrote "render" not "screenshot", which will be independent of graphics chip. Thank you, Jon! However, I just tried render and that seems to work as well. Did you install CCP4MG before or after upgrading to Catalina? Or was this a new computer o

[ccp4bb] difficult molecular replacement solution

I have been working on a protein structure which has been hard to solve by molecular replacement. Unit cell: (60.6, 172.34, 196.42, 90, 90, 90) Space group: P 21 21 21 The problem is that the homologues have only ~20% identity, and there are multiple chains in the asymmetric unit. The question

Re: [ccp4bb] difficult molecular replacement solution

Look at the chain packing in your solution. If the solution is grossly correct you will notice nice solvent channels and no glaring chain-size holes and no gross overlaps. The initial maps should look sensible, if noisy. If the protein is composed of likely dimers, try a search with dimer units t

Re: [ccp4bb] difficult molecular replacement solution

Hi Rob, the answer to the question "how many molecules are in the ASU?" is only available after satisfactory model building and refinement. The Phaser results that you show indeed suggest 6 mols/ASU, but they don't prove it. If I were you, I'd give the 6-molecule solution the highest priority,

Re: [ccp4bb] difficult molecular replacement solution

Have you looked at a self-rotation function? Those can sometimes be very useful in deciphering multiplicity. On 2019-11-13 09:33, Robert S Phillips wrote: I have been working on a protein structure which has been hard to solve by molecular replacement. Unit cell: (60.6, 172.34, 196.42, 90, 90,

Re: [ccp4bb] difficult molecular replacement solution

Dear Rob, Based on our experience with difficult MR cases, I recommend performing refinement on the coordinates from MR (I prefer PHENIX simulated annealing for this step). Then send the refined map - WITHOUT the model - to automated building with density modification. The auto-built model may

Re: [ccp4bb] difficult molecular replacement solution

I like to try molrep with something missing from the search model so that I can see if the resulting map includes density for something that I know should be there but the computer doesn’t. As others have pointed out, it also bolsters believability if the result agrees with a self-rotation func

Re: [ccp4bb] difficult molecular replacement solution

I am interested to know the resolution on the basis that the better it diffracts, the lower the solvent content, generally. Have you tried Bernard Rupp's server which gives you probabilities for the No mols per AU, partly based on resolution? The LLGs look challenging - I've seen higher ones refine

Re: [ccp4bb] difficult molecular replacement solution

Well, trying to solve a MR structure with low homology (20%) and several molecules is always a challenge... Use any information you have. How many molecules are possible/probable given the mol.wt and unit cell? (Matthews coeff gives suggestions..) But remember solvent content can vary from 30% to 7

[ccp4bb] Deadline Extended to December 9th

Hi CCP4BB, Thanks for sharing information about the AIMS Awards with your colleagues! Many have told us they really appreciated it. We’ve had requests for more time - so we extended the deadline to December 9th! For anyone with a protein target of interest, the AIMS Award program can help you di

[ccp4bb] Post-doctoral position in chaperone structural biology in Paris

Dear All, Please find bellow the announcement for a post-doctoral positon available in Paris. Best regards, Philippe A post-doctoral position is available to study the structural basis of chaperone-dependent protein translocation in the Laboratory of Molecular and Cellular Biology of Eukary

[ccp4bb] Scientist job position at AbbVie

Hi - I'd like to draw attention of interested candidates to the following job opportunity with full description to apply at: http://abbvie.referrals.selectminds.com/jobs/senior-scientist-i-ii-protein-x-ray-crystallographer-12821 Company overview: AbbVie (NYSE:ABBV) is a global, research-driven bi