Look at the chain packing in your solution. If the solution is grossly correct you will notice nice solvent channels and no glaring chain-size holes and no gross overlaps. The initial maps should look sensible, if noisy.
If the protein is composed of likely dimers, try a search with dimer units to simplify the search. Good luck. __________________ Roger Rowlett On Wed, Nov 13, 2019, 10:33 AM Robert S Phillips <p...@uga.edu> wrote: > I have been working on a protein structure which has been hard to solve by > molecular replacement. > > Unit cell: (60.6, 172.34, 196.42, 90, 90, 90) > Space group: P 21 21 21 > > The problem is that the homologues have only ~20% identity, and there are > multiple chains in the asymmetric unit. The question is how many. It > could be 4, 5, or 6 chains. > > N solvent P > 4 0.602 3.09 0.225 > 5 0.502 2.47 0.388 > 6 0.403 2.06 0.229 > > I have run PHASER with 4, 5 and 6 chains. I allowed it to search all > possible space groups, and P212121 was the best solution. These are the > results. > > N LLG TFZ > 4 104.9 7.5 > 5 137.5 7.7 > 6 166.2 8.3 > > Am I correct to conclude that there are 6 chains in the asymmetric unit? > > Rob > > Robert S. Phillips > Professor of Chemistry and of Biochemistry and Molecular Biology > University of Georgia > Athens, GA 30602 > Phone: (706) 542-1996 > Fax: (706) 542-9454 > E-mail: rsphill...@chem.uga.edu > Web: http://tryptophan.net > <https://pod51004.outlook.com/owa/redir.aspx?C=ccbf42ffea5f48b1bf8e9bb950454bab&URL=http%3a%2f%2ftryptophan.net> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
