Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread Patrick Shaw Stewart
I take the view that I'm trying to communicate with as many people as possible, without distracting them with my spelling . . . So go for US spellings. Sent from mobile On Tue, 23 Jul 2019, 22:39 Goldman, Adrian, wrote: > ..and responding in the same vein: > > my OED says that its etymology als

Re: [ccp4bb] Postdoctoral position in structural biology, Ghent University and VIB, Belgium

2019-07-24 Thread Kenneth Verstraete
Dear all, just a reminder about the post-doctoral position below. Interested candidates are encouraged to apply via https://jobso.id/bys2 . The deadline for application is August, 1st.  Kind regards, Kenneth Verstraete VIB and Ghent University, B

Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread CCP4BB
Hi First off, Tom is right - in science we should have standards (though don't get me onto propanone, ethanoic acid [obviously named after Ethan Merritt], spirits of salt, oil of vitriol etc). There ain't no such thing as Latin singular or Latin plural! Latin nominative singular or nominative

[ccp4bb] industrial PhD opportunity

2019-07-24 Thread Petri Kursula
Dear all, we currently have a possibility for carrying out an industrial PhD project with a successful local biotech company in Bergen (Norway); to this end, a 6-month position is open to get the project started and apply for industrial PhD funding from the Norwegian Research Council. The company t

[ccp4bb] MX Industrial Liaison Scientist position at Diamond Light Source

2019-07-24 Thread jitka.water...@diamond.ac.uk
Dear all, The Industrial Liaison group at Diamond Light Source currently offers an exciting opportunity to join our team. We are looking for a passionate and experienced structural biologist capable of contributing to the entire experiment from crystallisation to data analysis and model building

Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread Hargreaves, David
Dear CCP4bb, From my industrial perspective: The crystallisation bottleneck has probably been fairly well addressed. High throughput screening using robotics and around 20ul of protein per screen is common. There are lots of screens to try even if they are rather redundant in their content. How

[ccp4bb] Questionable Ligand Density - Part 2

2019-07-24 Thread Rhys Grinter
Hi All, Thanks for all the helpful comments and discussion surrounding my last post. I've been doing a little more investigation into this issue and wanted to see if people were able to provide me with some additional opinions/insights. I investigated the PDB entries for the lead deposition autho

Re: [ccp4bb] Questionable Ligand Density - Part 2

2019-07-24 Thread Rhys Grinter
Correction! I mean't 6BV0 et. al. Many apologies!!! Thanks Paul Brear for pointing this out. Rhys On Wed, 24 Jul 2019 at 21:36, Rhys Grinter wrote: > Hi All, > > Thanks for all the helpful comments and discussion surrounding my last > post. I've been doing a little more investigation into this

Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread Patrick Shaw Stewart
I have regularly been struck by how closely related proteins crystallize in completely different conditions. Eg see Galina Obmolova and colleagues' excellent paper (ref below). A set of sixteen highly homologous Fabs were crystallized in apparently random conditions. Roughly half used PEG, half h

Re: [ccp4bb] higher older tNCS

2019-07-24 Thread Rezaul Karim
Hello Jessica,You are working on MR, but tNCS option comes during refinement. In phaser, if you provide 3 copy or 3 components during MR setup, does not it work? Phenix online documentation should be the most updated. Thanks,Reza Sent from Yahoo Mail on Android On Tue, Jul 23, 2019 at 11:17

Re: [ccp4bb] Questionable Ligand Density - Part 2

2019-07-24 Thread Roger Rowlett
Question: While it is perhaps understandable that reviewers may not always have sufficient information to evaluate the quality of the interpreted model (and must trust that authors have acted in good faith in representing omit maps, etc.), is it not reasonable to expect reviewers to comment on

Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread Keller, Jacob
Yes, imagine 1 hr, tabletop, unlimited-length, error-free, dirt-cheap gene synthesis! (With a parallized option for 96-well plates, of course...) We'll probably just have to wait 10 years. JPK + Jacob Pearson Keller Research Scientist / Looger Lab

Re: [ccp4bb] Questionable Ligand Density - Part 2

2019-07-24 Thread Bernhard Rupp
Dear Ligand Hunters, Chris Weichenberger and I deposit a yearly update of ligand metrics, authors, journals, etc. of all ligands in the PDB. There is a huge spreadsheet you can transform into a Table and sort as desired, and also a basic applet to immediately view them in Coot. http://www.r

Re: [ccp4bb] Questionable Ligand Density - Part 2

2019-07-24 Thread Robbie Joosten
@4) Ligand density is a social construct in which the privileged blue en green density suppress the red density. The analogy of the nobility and the bourgeoisie suppressing the worker is completely obvious ;) Cheees, Robbie On 24 Jul 2019 20:13, Bernhard Rupp wrote: Dear Ligand Hunters, Ch

Re: [ccp4bb] challenges in structural biology

2019-07-24 Thread Artem Evdokimov
Unlimited length in an hour is physically unlikely, but the rest is not that far off I think. We are at or below 1 cent per basepair already (in bulk)... Artem On Wed, Jul 24, 2019, 13:01 Keller, Jacob wrote: > Yes, imagine 1 hr, tabletop, unlimited-length, error-free, dirt-cheap gene > synthe