Dear CCP4bb, From my industrial perspective: The crystallisation bottleneck has probably been fairly well addressed. High throughput screening using robotics and around 20ul of protein per screen is common. There are lots of screens to try even if they are rather redundant in their content. However, I feel there are two things missing: high throughput protein production at microscale and specific tools for construct design. Generally, industry is interested in the druggable site which perhaps is only one domain of a multidomain target protein. Getting a suitably robust crystal system often requires more than one iteration of multiple construct design which in my experience is something of a lottery. Some helpful design tools and an order of magnitude increase in the number of constructs that can be delivered from protein supply would help.
Best wishes, David Dr. David Hargreaves Associate Principal Scientist _____________________________________________________________________ AstraZeneca Discovery Sciences, Structure & Biophysics Dr David Hargreaves. Office R1-09/Lab FL54 AstraZeneca Darwin Building Unit 310 Cambridge Science Park Milton Road Cambridge CB4 0WG telephone: +441223223546 David.Hargreaves @astrazeneca.com Please consider the environment before printing this e-mail ________________________________ AstraZeneca UK Limited is a company incorporated in England and Wales with registered number:03674842 and its registered office at 1 Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0AA. This e-mail and its attachments are intended for the above named recipient only and may contain confidential and privileged information. If they have come to you in error, you must not copy or show them to anyone; instead, please reply to this e-mail, highlighting the error to the sender and then immediately delete the message. For information about how AstraZeneca UK Limited and its affiliates may process information, personal data and monitor communications, please see our privacy notice at www.astrazeneca.com<https://www.astrazeneca.com> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
