[ccp4bb] EMBL Workshop "Cryo-EM in Industry & Academia", Heidelberg, 6-8 February 2019

2018-10-19 Thread Christoph Mueller
Dear Colleagues, This is to invite you to the workshop "Cryo-EM in Industry & Academia", that we are organizing at EMBL Heidelberg, 6-8 February 2019. The workshop will focus on success stories and new technologies, but also on challenges industry is facing using cryo-EM. The workshop is equal

Re: [ccp4bb] viewhkl : misleading intensity appearance ?

2018-10-19 Thread Eleanor Dodson
As you say, the two are related but look different, and the intensity on the subsequent images look different too However there are some default scaling parameters, and I have no idea what they are.. Any alternating strong/weak pattern should generate a strong off-origin peak in the native Patters

[ccp4bb] ARP/wARP-8.0 + CCP4-7.0.065 joint release

2018-10-19 Thread Andrey Lebedev
Dear CCP4 Users We would like to announce the joint release of ARP/wARP version 8.0 and CCP4-7.0.065. ARP/wARP 8.0 provides a considerably improved performance for X-ray crystallographic applications and it is now also capable of interpreting cryo-EM maps at a resolution range 2.0-3.5 Å. Install

[ccp4bb] 50% TA position at U. Konstanz

2018-10-19 Thread Olga Mayans
Dear colleagues, We have a part-time opening for a technical assistant in our laboratory at the University of Konstanz. The position might be of interest to candidates in our geographical area. Please kindly bring it to the attention of suitable applicants in your lab. Full details at: https:

[ccp4bb] Computational cryo-EM faculty position at the Morgridge Institute for Research and UW-Madison

2018-10-19 Thread Eric Montemayor
Dear Colleagues, A great faculty-level opportunity between the Morgridge Institute for Research and the University of Wisconsin-Madison. We seek to hire a tenure-track assistant/associate/full professor in the broad area of cryo-EM centered quantitative image processing and analysis. This position

[ccp4bb] suggestions for cryoprotectant

2018-10-19 Thread Firdous Tarique
Dear members I have got beautiful crystal hits in SaltRx screens which are not diffracting to a good resoultion. All of them are salt based condition and I am not able to formulate a good cryoprotectant for these crystals. I also think that in my case the poor resolution is due to a poor cryoprote

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-19 Thread Pascal Egea
Hi Firdous, I noticed that conditions 2,4 and 5 are overall harsh agents (chaotropic agents such as KSCN (probably not KCN) and Nitrate ions ), in my own personal experience I have never been very lucky with crystals grown in nitrate ions. Condition 1 however looks a bit more soft with 'gentle' io

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-19 Thread Christine Gee
Dear Fridous Have a look at https://www.ncbi.nlm.nih.gov/pubmed/14646118 Malonate as a cryoprotectant Or https://www.ncbi.nlm.nih.gov/pubmed/20606259 Regards Christine On Fri, Oct 19, 2018 at 2:57 PM Firdous Tarique wrote: > Dear members > > I have got beautiful crystal hits in SaltRx screens

[ccp4bb] RapiData at SSRL 2019

2018-10-19 Thread Smith, Clyde
Hi everyone, Applications are now open for the 2019 edition of RapiData at SSRL. RapiData is a practical course in macromolecular X-ray diffraction data collection, data processing and structure solution. The aim of the RapiData course is to educate and train young scientists in data collection