[ccp4bb] Guard columns from FPLC

2014-09-16 Thread Anita P
Hi All, Sorry for this off topic. I have heard that there are these little columns called guard columns which can be attached to AKTA purifiers. These columns prevent the incoming huge aggregates to be deposited and blocking of the gel filtration columns. Can any one advice me regarding where to

[ccp4bb] Announcement of Workshop "Fragment-Screening by Crystallography"

2014-09-16 Thread Müller , Uwe
Dear all, as a satellite to the 2014 HZB User Meeting, which will take place from Dec 03-05, 2014 in Berlin, we are organizing a one-day workshop on "Fragment-Screening by Crystallography". We assembled a number of highlight presentations from both academia and industry in order to give an overview

Re: [ccp4bb] Guard columns from FPLC

2014-09-16 Thread Christian Roth
Hi Anita, never heared about Guard columns for Superdex columns. These guard columns are usually for HPLC columns (RP) or Silica High pressure gelfiltration columns like from Tosoh Bioscience and part of the acutal column itself, though replaceable. Christian Am 16.09.2014 09:29, schrieb An

Re: [ccp4bb] Guard columns from FPLC

2014-09-16 Thread Nadir T. Mrabet
Well, Pharmacia (now, GE) used to sell such a guard column for FPLC prior to Akta. And I remember buying adaptors to be able to connect the guard column and FPLC columns to an HPLC system. So all the tools exist. But keep in mind that a guard column volume must in all cases be kept minimal to a

Re: [ccp4bb] Guard columns from FPLC

2014-09-16 Thread Scott Pegan
Anita, >From your description, you most likely are looking for a prefilter6000. http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences/18458201 Scott Scott D. Pegan, Ph.D. Associate Professor Pharmaceutical and Biomedical Sciences College of Pharmacy University o

Re: [ccp4bb] Guard columns from FPLC

2014-09-16 Thread Katherine Sippel
I think the typical Akta configuration has the prefilter installed after the mixing pump, but before the sample loop. That is where it is on mine at least. It wouldn't stop sample precipitate from loading on the column. Technically, it would probably still work if you installed the prefilter after