Well, Pharmacia (now, GE) used to sell such a guard column for FPLC
prior to Akta.
And I remember buying adaptors to be able to connect the guard column
and FPLC columns to an HPLC system.
So all the tools exist.
But keep in mind that a guard column volume must in all cases be kept
minimal to avoid sample dilution which can be deleterious to resolution
in size-exclusion chromatography.
Prior to FPLC with (or without) a guard column, I have always used an
Eppendorf microcentrifuge followed by 2u-filtering prior to injecting
the sample and have consistently observed that these remove most
aggregates if not all.
HTH,
Nadir Mrabet
Pr. Nadir T. Mrabet
Structural & Molecular Biochemistry
N-gere - INSERM U-954
University of Lorraine, Nancy
School of Sciences and Technologies
& School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet <at> univ-lorraine.fr
Cell.: +33 (0)6.11.35.69.09
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On 16/09/2014 19:01, Christian Roth wrote:
Hi Anita,
never heared about Guard columns for Superdex columns. These guard
columns are usually for HPLC columns (RP) or Silica High pressure
gelfiltration columns like from Tosoh Bioscience and part of the
acutal column itself, though replaceable.
Christian
Am 16.09.2014 09:29, schrieb Anita P:
Hi All,
Sorry for this off topic.
I have heard that there are these little columns called guard columns
which can be attached to AKTA purifiers. These columns prevent the
incoming huge aggregates to be deposited and blocking of the gel
filtration columns.
Can any one advice me regarding where to purchase these columns. I could
not find them in GE website. We have Superdex 16/60 on AKTA purifier.
Thanks in advance.
Have a good day
Anita
