I think the typical Akta configuration has the prefilter installed after the mixing pump, but before the sample loop. That is where it is on mine at least. It wouldn't stop sample precipitate from loading on the column. Technically, it would probably still work if you installed the prefilter after the sample loop, but that would add to the dilution problem stated earlier.
As mentioned by a previous commenter, I personally spin filter everything right before I load it. That is usually sufficient unless the buffer change is causing the protein to crash out. Of course if that is the case a guard column not the solution. Cheers, Katherine On Tue, Sep 16, 2014 at 12:46 PM, Scott Pegan <scott.d.pe...@gmail.com> wrote: > Anita, > > From your description, you most likely are looking for a prefilter6000. > > > http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences/18458201 > > Scott > > > Scott D. Pegan, Ph.D. > Associate Professor > Pharmaceutical and Biomedical Sciences > College of Pharmacy > University of Georgia > 240 W. Green St. > Athens, GA, USA, 30602 > phone: 706-542-3435 > > On Tue, Sep 16, 2014 at 4:29 AM, Anita P <crystals...@gmail.com> wrote: > >> Hi All, >> Sorry for this off topic. >> >> I have heard that there are these little columns called guard columns >> which can be attached to AKTA purifiers. These columns prevent the incoming >> huge aggregates to be deposited and blocking of the gel filtration columns. >> >> Can any one advice me regarding where to purchase these columns. I could >> not find them in GE website. We have Superdex 16/60 on AKTA purifier. >> >> Thanks in advance. >> Have a good day >> >> Anita >> > > -- "Nil illegitimo carborundum"* - *Didactylos
