*Postdoctoral Fellowships: Structural Biology / Molecular machines in
protein targeting and ribosome biogenesis*
The lab of Prof. Irmi Sinning at the Biochemistry Center (BZH) of
Heidelberg University seeks to recruit 4 outstanding postdoctoral
researchers for a period of 2--5 years.
BZH is
The Canadian Macromolecular Crystallography Facility (CMCF) is excited to
announce an intensive 5-day hands-on synchrotron data collection school at the
Canadian Light Source (CLS) in Saskatoon. The School will take place June 5 -
9, 2012. Participants will attend a series of lectures and be act
Hi all,
I have a vague memory of having a picture in someone's presentation once,
showing a smoking hot X-ray beam emerging from the beam pipe in a hutch at a
synchrotron. I think the picture might have been a double exposure, with a long
exposure that captured air ionization superimposed on a
Dear all,
Sorry about a non-crystallographic question here.
I am working on a kinase and would like to get an ATP analogue into
the crystals. When soaked with AMP-PCP, the kinase crystals crack in
about 15 min at 4 C.
I could try other analogues like AMP-PNP etc, but those would probably
behavou
Hi Pat -
I, too, have a memory of such a picture. This isn't quite the one I was
thinking of, but it should hopefully serve the purpose:
http://www.nsls.bnl.gov/about/imagelibrary/images/hr/Synchrotron_Light2_hires.tif
(there are a couple more from the parent page:
http://www.nsls.bnl.gov/a
On 2/1/12 2:34 PM, Matthew Franklin wrote:
Hi Pat -
I, too, have a memory of such a picture. This isn't quite the one I
was thinking of, but it should hopefully serve the purpose:
http://www.nsls.bnl.gov/about/imagelibrary/images/hr/Synchrotron_Light2_hires.tif
(there are a couple more f
On Wed, Feb 1, 2012 at 11:17 AM, Dianfan Li wrote:
> I am working on a kinase and would like to get an ATP analogue into
> the crystals. When soaked with AMP-PCP, the kinase crystals crack in
> about 15 min at 4 C.
This isn't too surprising; most kinases undergo global conformational
changes (dom
Dianfan
Some kinases have such conformation in non-activated apo form that the ATP
binding site is partially obstructed. Soaking an ATP analog may then have 3
outcomes: 1) successfully open up the binding site without damage to the
crystal, 2) fail to open up the active site and the compound ca
On Feb 1, 2012, at 12:17 PM, Dianfan Li wrote:
> I am working on a kinase and would like to get an ATP analogue into
> the crystals. When soaked with AMP-PCP, the kinase crystals crack in
> about 15 min at 4 C.
15 minutes is a long time. Scoop crystals during that time period.
Do the cracked
I assume that cocrystallization has failed? What you are experiencing is
likely the effect of conformational transition caused by ligand. You can
try very slow adition (even microdialysis) or if your ligand is fairly
insoluble then you can just add a tiny solid particle of inhibitor to your
drop an
Dear Patrick,
One such image I can remember was that of the first beam at ESRF. If
you Google for "ESRF first beam", asking for Images, the first one you get
(top left corner) is rather similar. The original one was, I think, more
dramatic than that (or perhaps I was more impressionable). It
Hi,
First, it's very much a crystallographic question. Second, the success or
failure in soaking in ligands/cofactors depends quite often also to the crystal
packing. Some packing forms (and the spacegroups that go with it) will tolerate
the soaking even if it's accompanied with a conformation
Hi ,
To add to the previous comments,
crystallization of GTP or ATP (or their analogues) with their kinase/ A- or
G-tpases can depend on a lot of factors that were mentioned (such as
packing).
A simple common problem is that ATP solutions should be carefully buffered
prior to their use for soakin
Dear Dianfan,
In some cases the ATP-lid of the kinase is blocking the active site in the
crystal form. In those cases the only option is to try co-crystallisation.
Besides ATP and the homologs you mention you can also try ADP that as you will
see in the PDB has been heavily used for kinases.
B
Maybe someone has suggested this already... If so, I am re-enforcing it.
If the cracking is coming from actual molecular movement induced by binding
(and not other reason like differing ionic strength in your soaking conditions)
you could try setting up some co-crystallization and (hopefully)
gr
Dear All,
For the 3.4 M sodium malonate of Hampton Research, will you please tell me how
the pH was regulated to 6.0, 7.0 and 8.0 separately?
Cheers,
Dialing
Dialing,
Malonic acid is dissolved in water and then pH adjusted to the desired value
with NaOH. Caution: dissolving malonic acid is highly exothermic. Do it slowly,
in a hood.
Doug
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dialing
Pretty
Sent: Wednesday,
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