Dear all, Sorry about a non-crystallographic question here.
I am working on a kinase and would like to get an ATP analogue into the crystals. When soaked with AMP-PCP, the kinase crystals crack in about 15 min at 4 C. I could try other analogues like AMP-PNP etc, but those would probably behavour in a same way as AMP-PCP. Is it a good idea of trying quick soaks at high concentrations of AMP-PCP? Co-crystallization is another option I have but AMP-PCP is a substrate of the kinase (with low rate). What are other ways of getting ATP analogues into a crystal? Thanks for suggestions, Dianfan Dianfan Li, PhD College of Biochemistry and Immunology Trinity College Dublin Dublin, Ireland.
