I assume that cocrystallization has failed? What you are experiencing is likely the effect of conformational transition caused by ligand. You can try very slow adition (even microdialysis) or if your ligand is fairly insoluble then you can just add a tiny solid particle of inhibitor to your drop and hope it dissolves slowly and saturates the crystal slowly. Hope it helps :-) Artem On Feb 1, 2012 1:31 PM, "Dianfan Li" <l...@tcd.ie> wrote:
> Dear all, > > Sorry about a non-crystallographic question here. > > I am working on a kinase and would like to get an ATP analogue into > the crystals. When soaked with AMP-PCP, the kinase crystals crack in > about 15 min at 4 C. > > I could try other analogues like AMP-PNP etc, but those would probably > behavour in a same way as AMP-PCP. Is it a good idea of trying quick > soaks at high concentrations of AMP-PCP? Co-crystallization is another > option I have but AMP-PCP is a substrate of the kinase (with low > rate). > > What are other ways of getting ATP analogues into a crystal? > > Thanks for suggestions, > > Dianfan > > Dianfan Li, PhD > College of Biochemistry and Immunology > Trinity College Dublin > Dublin, Ireland.
