Or maybe a problem of oxidation?
I had a Se-met crystal that could be crystallized and diffracted just like the
native one, but only in the presence of the reducing agent (TCEP), no crystal
otherwise.
Tc
寄件人﹕ Frederic VELLIEUX
收件人﹕ CCP4BB@JISCMAIL.AC.UK
傳送日
Don't forget to do seeding with the native crystals: it's often more about
nucleation than growth. Phx
Sent from tiny silly touch screen
- Reply message -
From: "Frederic VELLIEUX"
Date: Mon, Jun 13, 2011 07:37
Subject: [ccp4bb] Fwd: Regarding Sel-Met containing proteing crystallisatio
Dear All,
does anyone know of a program that can measure the volume or
largest dimension of the solvent channels in crystals? Cheers, Matt.
--
Matthew Bowler
Structural Biology Group
European Synchrotron Radiation Facility
B.P. 220, 6 rue Jules Horowitz
F-38043 GRENOBLE CEDEX
FRANCE
==
It may require some tricks, such as the creation of walls of CA atoms, but
Kleywegt's FLOOD will give an answer in terms of number of water molecules.
Anthony Duff
-Original Message-
From: CCP4 bulletin board on behalf of Matthew BOWLER
Sent: Mon 6/13/2011 5:40 PM
To: CCP4BB@JISCMAIL.A
Dear Atul,
Whilst finding Se-Met conditions you could always just phase with mercury if
you are lucky enough to have an accessible free Cys residue or try NaI or I3C
soaks. Always worth trying if you have readily reproducible crystals…
Best of luck
Marc
From: atul kumar mailto:atulsingh21...
On 06/13/2011 04:55 PM, DUFF, Anthony wrote:
It may require some tricks, such as the creation of walls of CA atoms,
but Kleywegt's FLOOD will give an answer in terms of number of water
molecules.
Some more tools are referenced here:
http://hollow.sourceforge.net/
However, I did not tried them.
Postdoc opening at the MRC Laboratory of Molecular Biology, Cambridge, UK.
Murray Stewart's Structural Cell Biology group at the MRC Laboratory of
Molecular Biology in Cambridge is seeking a postdoctoral scientist
(3-year Career Development Fellow position) to work on the structural
basis of t
Hallo Matthew,
you can also mark the diameters with some atoms (e.g. water molecules in coot),
cut them out and use moleman2 ("xyz align" to align the object and "stats" to
get the radii of gyration) as a rough estimate.
Tim
On Mon, Jun 13, 2011 at 09:40:47AM +0200, Matthew BOWLER wrote:
> Dear A
Thank you to all!
@Frederic
>
> I have a problem with the following sentence:
> "if I collect all spots I get good map, but it is impossible to solve the
> structure by molecular replacement" - if you have a good map (I assume
> electron density map) then the structure is solved... for me a goo
"the space group is I422" "do you have any other suggestion?"
Yes, how certain are you of the space group? For myself, I'm never
entirely certain of the space group until I have solved the structure...
I always keep in mind the other possibilities for space group
assignment, if need be. And so
On Mon, Jun 13, 2011 at 9:48 AM, Dhanasekaran Varudharasu <
dhana...@gmail.com> wrote:
> We have solved one protein structure with barium as
> a heavy atom using SAD technique. But in the final refinement one barium
> site shows split. But the distance between the two posit
Given that the water and the barium seem to have the same electron density
on image electron
png Id look careful at relative density levels,
occupancies, B-factors etc. Often the situation becomes clearer during later
stages of refinement.
Peripherally, I also do not exactly understand the di
Hi.
I was considering using GTP Agarose Resin for the final clean up step of the
purification of a GTPase and was wondering if anyone has had experience
using this resin. My main concerns are whether it actually has a decent
binding capacity for GTP binding proteins, considering that endogenous
G
Hi Matthew,
Most GTPases required Magnesium to hydrolyze so you maybe able to reduce
this by working in presence of EDTA and absence of magnesium. This may
promote removal of traces of GTP or GDP ( from previous experience with SRP
GTPases I would be more worried about residual GDP). Having EDTA a
Dear colleagues,
I would like to draw your attention to an upcoming free, educational webinar to
be presented by Angela Criswell, Ph. D. titled "Small Angle X-ray Scattering
(SAXS) Techniques for Macromolecules". Small angle X-ray scattering is a
technique used to study dilute solutions of macr
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