Sorry Ed,
We will add that function to the server in a few days.
Fei
On Wed, 27 Apr 2011 13:49:17 -0400, Ed Pozharski
wrote:
> Is there any way to download all of the files produced by a BALBES run
> on YSBL server at once (as opposed to going through every item
> individually)?
>
> A relat
Dear BB readers,
at the University of Milan we are currently looking for a motivated candidate
for a PhD student position in the lab of Prof. Bolognesi, under Bolognesi's and
my supervision.
The project will be focusing on crystallographic and biophysical studies of
Beta-2 microglobulin, an a
Title: DNA oligonucleotide purification
All,
Our laboratory is looking for a new column to purify short (40mers or less) DNA oligonucleotides that will be used for crystallization setups. I wanted to ask if anyone has suggestions of a column that will give excellent separation of N and N-1 prod
Hi Jamie -
We've had good success with these columns from Dionex (the DNAPac PA-100 and
the DNAPac PA200 (Analytical 4x250mm and Semi-Prep 9x250mm,)). We use them
precisely for the application you describe.
http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa100/l
p-
Hi everyone,
This is although an off topic question but I would certainly seek expert
advices on the following query:
I have a GST-tagged protein that is purified in presence of the steroids. I
carry out an in column digestion using thrombin. Digestion buffer
compositin:
50mM Tris pH: 8.0,
150m
Title: Re: [ccp4bb] DNA oligonucleotide purification
Kushol,
Thank you for the reply. Might I ask what kind of HPLC system you have? Our HPLC is old and has stainless steel pumps, and to our knowledge this is not compatible with the Dionex columns (due to metal leaching). Dionex has recommended
We're using an older Dynamax system that is outfitted with PEEK throughout.
Cheers,
Kushol
From: Wallen, Jamie [mailto:jwal...@biochem.wustl.edu]
Sent: Thursday, April 28, 2011 10:17 AM
To: Kushol Gupta; CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] DNA oligonucleotide purification
Kus
We actually screen with unpurified oligos (up to just over 30nt) and it works
just fine. Saves lots of time & $$.
If we get hopefull crystals, we pay for purification or do it ourselves by
gel. Sometimes it makes the crystals better and sometimes it doesn't.
Phoebe
=
Hmm - I use this quite a bit.
ifier.
You cant start Autoamore for a particular model until that has been
entered into the model database - the first Q you are asked from the GUI
is "Which model?" But you can certainly close the model database once
you have entered the model name with a unique
I installed a fresh copy of FC14 at home and am having problems with my CCP4
installation. When I try to input an mtz file into any CCP4i program, I get the
following errors:
Error in LABIN: label Unassigned not found in file!
MOLREP(ccp4): Error in label assignments in LABIN
MOLREP(ccp4): Er
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