We're using an older Dynamax system that is outfitted with PEEK throughout.
Cheers, Kushol From: Wallen, Jamie [mailto:jwal...@biochem.wustl.edu] Sent: Thursday, April 28, 2011 10:17 AM To: Kushol Gupta; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] DNA oligonucleotide purification Kushol, Thank you for the reply. Might I ask what kind of HPLC system you have? Our HPLC is old and has stainless steel pumps, and to our knowledge this is not compatible with the Dionex columns (due to metal leaching). Dionex has recommended either a titanium or peek system. As our HPLC is still running well, we were hoping to find a column that can tolerate the stainless steel. Jamie On 4/28/11 9:11 AM, "Kushol Gupta" <kushol.gu...@gmail.com> wrote: Hi Jamie - We've had good success with these columns from Dionex (the DNAPac PA-100 and the DNAPac PA200 (Analytical 4x250mm and Semi-Prep 9x250mm,)). We use them precisely for the application you describe. http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa100/l p-73370.html http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa200/l p-73371.html cheers, Kushol Kushol Gupta, Ph.D. Research Associate Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine kgu...@mail.med.upenn.edu <BLOCKED::mailto:kgu...@stwing.upenn.edu> 215-573-7260 / 267-259-0082 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Wallen, Jamie Sent: Thursday, April 28, 2011 9:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] DNA oligonucleotide purification All, Our laboratory is looking for a new column to purify short (40mers or less) DNA oligonucleotides that will be used for crystallization setups. I wanted to ask if anyone has suggestions of a column that will give excellent separation of N and N-1 products. We have a Shimadzu HPLC system. We are considering Dionex columns as well as Oligonucleotide Separation C18 columns from Waters, but if there are others that are better we would like to know. Any suggestions will be greatly appreciated. Thank you! Jamie Wallen
