Hi Jamie -
We've had good success with these columns from Dionex (the DNAPac PA-100 and the DNAPac PA200 (Analytical 4x250mm and Semi-Prep 9x250mm,)). We use them precisely for the application you describe. http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa100/l p-73370.html http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa200/l p-73371.html cheers, Kushol Kushol Gupta, Ph.D. Research Associate Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine <BLOCKED::mailto:kgu...@stwing.upenn.edu> kgu...@mail.med.upenn.edu 215-573-7260 / 267-259-0082 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Wallen, Jamie Sent: Thursday, April 28, 2011 9:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] DNA oligonucleotide purification All, Our laboratory is looking for a new column to purify short (40mers or less) DNA oligonucleotides that will be used for crystallization setups. I wanted to ask if anyone has suggestions of a column that will give excellent separation of N and N-1 products. We have a Shimadzu HPLC system. We are considering Dionex columns as well as Oligonucleotide Separation C18 columns from Waters, but if there are others that are better we would like to know. Any suggestions will be greatly appreciated. Thank you! Jamie Wallen
