Sankar,
All the executables you downloaded (including bltwish) from the CCP4
site are compiled on a 32bit machine. You just need to install the 32bit
shared library package via synaptic. The following terminal command should
do.
sudo apt-get ia32-libs
As for coot for what platform is your v
Try refinement with the newest REFMAC which will take twinning into
account.
But if your integration has missed some spots, or the spots were
streaky you may have more complex problems - with what is termed
Order-disorder in the lattice.
eleanor
Rana Refaey wrote:
Hi all,
I have a few
Cant you read the shell limits off the loggraphs? The numbers in the
tables are given as 4sin**2/Lamba**2 I think but that is converted to As
in the loggraph.
Is that what you mean though?
Eleanor
Francis E Reyes wrote:
Hello ccp4'ers
Where is / how can I obtain the resolution shell for each
The table has 1/d^2 and Dmin for the outer edge of each bin (columns 2
& 3)
Phil
By 4SINTH/LASQ bins (all statistics relative to Mn(I))
__
codebase="/usr/local/CCP4versions/Clapham/ccp4-6.1.1/bin">name="table" value="
$TABLE: Ana
Hi All,
I got some hexagonal crystals in one of my crystal condition. The protein is
a membrane protein and contains 0.05% DDM. Has anybody seen DDM crysals and
if yes, how do they look like?
thanks in advance
Parveen Goyal
Why is the tantalum bromide cluster the cluster derivative of
choice for phasing large structures? As a inorganic chemist
(in a previous incarnation) I can think of a lot of other
possible clusters that might be suitable. I even have money
from a bioinorganic grant that could finance a PhD student
Hi Parveen,
Not a direct answer to your question but we could possibly help by
X-raying the crystals in-situ whilst protected and undisturbed in the
multi-well plate. This should definitively show whether they are protein
or detergent crystals.
Depending on where you are located, we may have a
Hi Parveen.
DDM crystals can look a bit like that. Like a pencil that has been
sharpened from both ends until there is very little pencil left, but often
the ends are flatter than that. You can get tons of these (also with DM) in
conditions with medium weight PEGs. Of course many proteins will als
Hello CCP4BBers,
A permanent scientist position is open on the PROXIMA 2 beamline at
SOLEIL. Please see below for more information.
Cheers,
Bill
Beamline Scientist PROXIMA 2 (m/f)
(Ref: EXP-102)
SOLEIL is a state-of-the-art, third generation synchrotron radiation
centre dedicate
Hi Jose,
how do you know that those crystals were detergent and not protein? My
impression is that it is really hard to crystallize DDM, and even harder for DM
(solubilities > 20% in water). The easiest (?) way to check this may be to take
some crystals, wash them well and run them out on a PAG
Hi Jose,
It is quite difficult to crystallize DDM. The main problem is to estimate
detergent concentration in the final sample.
One method requires the use of chromatographic workstation coupled to a
light scattering detector and refraction index measurement unit. In this
case you can know if there
Parveen,
Bert and Pascal are correct in that most alkyl glycoside detergent are
notoriously difficult to crystallize in aqueous solution when you have
the beta-anomer (what we normally buy). However, the alpha-anomers
can be quite easy to crystallize and can contaminate batches of beta-
a
A recommendation: try looking at the crystals while rotating the polarizers.
Often you can get detergent or detergent-protein complex "crystals" which have
sharp edges, but are actually liquid crystals. This will be manifest as a
compact-disc (or vinyl LP, depending on your vintage) appearance w
I was hoping for a break down for each bin similar to that of d*trek..
for example:
Rmerge vs Resolution
Resolution AverageNum Num I/sig I/sig Rducd Model
Rmerge Rmerge
range counts rej
This has been elaborated before, but you can safely assume they are NOT
detergent crystals. DDM may be harder to crystallize than your average membrane
proteinI hope those crystals diffract!
Bert van den Berg
University of Massachusetts Medical School
Program in Molecular Medicine
Biotech II
Thank you very much for the advice Dr. Weeks,
I could run CCP4 GUI now with out any problem.
As you have rightly said, I downloaded the COOT along with CCP4 as a package
(generic linux x86 version).
I will try downloading the CENT OS version and follow the method from the
previous posts to r
AsCA'09 Beijing
Joint Conference of the Asian Crystallographic Association and Chinese
Crystallographic Society
October 22-25, 2009, Beijing, China
Website: http://www.ciccst.org.cn/asca09/
PLEASE NOTE: The deadline for submission of abstracts has been extended to
August 5, 2009.
SPONSORS:
The
17 matches
Mail list logo
