Hi Rebecca,
The citation I use is from the 2005 CCP4 study weekend:
Scaling and Assessment of Data Quality, Philip Evans, Acta Cryst D 62
72-82.
It is open access from www.iucr.org.
Cheers,
Graeme
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Be
It looks like as if your PDB is corrupt. For example there atoms B
that should not be present inamino acids.
Garib
On 9 Jul 2007, at 19:55, JINJIN ZHANG wrote:
Hello all,
I'm working on a protein-DNA complex. My protein is a trimer and
the crystal has 3 trimers in an AU. I used a pdb fi
Page, Rebecca escribió:
CCP4ers:
What is the correct reference for Phil Evans Program POINTLESS?
Thanks for your help!
Rebecca
Evans, P. (2006) Scaling and assessment of data quality. /Acta
Crystallogr. sect. D/, *62*, 72-82.
--
That's the right (& indeed only) one (although more & up-coming
versions are a little different to that described there)
Phil
On 10 Jul 2007, at 08:36, Winter, G (Graeme) wrote:
Hi Rebecca,
The citation I use is from the 2005 CCP4 study weekend:
Scaling and Assessment of Data Quality, Phil
Mary,
freezing habitually increases mosaicity. In your case, the high water
content adds to the problem.
Try not to freeze the crystal but collect at sub-zero temperature (in
short glass capillaries or use oil plugs instead).
You have to optimize the "close to freezing" data-collection tempera
Postdoctoral Training Fellow
Structural Biology of Hsp90 – Client Protein Complexes
Section of Structural Biology
The Institute of Cancer Research
Chester Beatty Laboratories
Chelsea, London
The Institute of Cancer Research is one of the world's leading cancer
research
organisations and is i
You might also want to loook into using parathone for freezing... Or
collection at 260K indeed!
Flip
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
mesters
Sent: Tuesday, July 10, 2007 11:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with re
Hi Mary,
There are many excellent suggestions already but you may want to try
tweaking your cryo a bit. This is described very nicely by Ed Mitchell
and Elspeth Garman in a paper on mosaic spread as a function of
cryoprotectants concentration - J. Appl. Cryst. (1994) 27, 1070-1074.
You already hav
==
Synchrotron Beam Time available for Macromolecular Crystallography
at Berkeley Center for Structural Biology Beamlines
(5.0.1, 5.0.2, 5.0.3, 8.2.1 and 8.2.2).
The ALS is now accepting Gen
Jeroen brings up a good point. Back in the old days, around 5 B. C.
(Before Cryo), we would use a chilled air generator to blow a stream
of cold air along the capillary axis to keep the crystals just above
their freezing point--it made a huge difference in crystal lifetime.
I recall a coll
CCP4 bulletin board wrote on 07/10/2007 02:07:37
PM:
> Jeroen brings up a good point. Back in the old days, around 5 B. C.
> (Before Cryo), we would use a chilled air generator to blow a stream
> of cold air along the capillary axis to keep the crystals just above
> their freezing point--it made
I have a Shelx HKLF4 formated reflection file that I would like to
convert to a mtz file. The primary reason is to transfer the freeR
set. I found a couple of convoluted ways of converting the
reflection data, but no real way of getting the freeR set to transfer.
Any suggestions?
Cheers
This notification is posted on behalf of Prof Wim Hol. Interested parties
should reply to him at:
[EMAIL PROTECTED]
or at the address below.
Postdoctoral Fellow Structural Biology of the RNA editing editosome
JOB DESCRIPTION:
The project aims at understanding the s
You can use f2mtz with the format identifier to convert the file:
8< snip-> script start <--
#/bin/bash
f2mtz hklin yourshelxfile.hkl hklout yourmtzfile.mtz << eof
cell"you cell dimensions"
symm "your space group"
format '(3F4.0,2F8.2,F4.0)'
ctypout H H H J Q R
LABOUT H K L
Hi,
I like to short out how I can see map in pymol and bobscript.
When I am using pymol, I cann't see map file. Same map file I can see in Coot.
Map file (created for O using from .fcf shelxpro) is also not coming;
extenstion .xplor (or .dsn6) not working either.
message:
Crystal: Unit
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