I second Chris's suggestions. These have worked well for me in the
past. You only need a very thin layer of the grease (i.e. keep
wiping until its almost completely gone) and it usually has no affect
on the crystallization.
Jeff
On Jan 27, 2009, at 3:51 PM, Christopher Colbert wrote:
Hi Savvas,
If the very good suggestions you have already got from the ccp4bb do not
help, try crystallization with agarose as an additive. Crystals form
inside the very soft gel and they are hold in place by this meshwork.
So, they are mechanically protected and do not fall down onto the bottom
of
Most obvious (maybe you did it already but that is not clear from your
email), why not try seeding?
- J. -
Savvas Savvides wrote:
Dear colleagues,
we have been growing crystals of a protein complex in sitting-drop
geometry that stick to the bottom of the drop remarkably well. It’s as
if t
Hi All
One more method that I heard about but never tried is to put the plate
on a sonication bath and to let for a short sonication pulse. That
should vibrate some liquid under your crystal.
Raz
--
Raz Zarivach, Ph.D.
Department of Life
Hi Savvas,
You can collect data on your crystal still in the drop, on our beamline
(FIP-BM30A at the ESRF) if you are interested. Provided space group is
not P1 We do that routinely.
Let me know if you are interested.
JL
Savvas Savvides wrote:
Dear
colleagues,
we
have b
Hi,
I had a similar story like yours.Then I added a drop of 10ul simulated mother
liquot which contains much higher concentrations of all components in the
normal mother liquot. Sometimes, the crystals attached to the plastic would
float to the surface. If not, take another 10ul, but blew it
Suggestions so far have been good ones. However, the MiTeGen microtools kit:
http://mitegen.com/products/microtools/microtools_kit1.shtml
comes with a "MicroSaw", which is a 10-micron thick kapton saw that is
intended for this purpose. That is, you don't pry the crystal off the
surface, but rat
If you have good and bad crystals in the same drop, I've had success
pushing a crummy crystal into a good crystal and having it release that
way.
Additionally, once I realized this was going to be a long term problem, I
started coating the sitting drop depressions with a thin layer of vacuum
g
Hi,
I had this exact problem before. The method below worked for me:
Take a sturdy needle (like one of the microneedles from a Hampton kit or a
very thin syringe needle) and (while observing the whole thing under a
scope) stick the needle into the plastic a bit away from the crystal. Push
h
Put a small piece of dry ice on the opposite side of the plastic from the
crystal. Perhaps the difference in thermal expansive coefficient will let
the crystal(s) break away. Don't overdo it though. This is a trick that
Gary Gilliland taught me.
Jim
On Wed, 28 Jan 2009, Savvas Savvides wro
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