Re: [ccp4bb] low UV reading on AKTA prime

2009-07-08 Thread Marius Schmidt
Yes, our new Akta prime has also only 2 mm path length and readings are, therefore, much smaller than with 1 cm path length in a typical spectro-photometer. Best Marius > Hi all, > > We recently got low UV reading on our AKTA prime. We contacted GE > healthcare, but was told that the UV reading

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-07 Thread Leiman Petr
chines fit perfectly into the AKTA UV unit and have a much longer life (15+ years) than the new GE-made filters. Best, Petr From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Patrick Loll Sent: Wednesday, July 01, 2009 11:18 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Lijun Liu
Hi Matt, AKTA system does allow you check the how-good percentage of the UV lamp, so if the UV lamp is new, and it gives low percentage (I do not mean the 20%) then your AKTA system may have the problem like Scott said or other thing in the Optical path. There are other possibilities, however! F

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Patrick Loll
I second Scott's post. About the only problem we've had with our Akta instruments is this type of degradation of the filter. I'm not sure what the mechanism is, but the filters do seem to crap out after a while, at least in the cold room (oxidation? I have no idea of what the filter is mad

Re: [ccp4bb] low UV reading on AKTA prime

2009-07-01 Thread Scott Walsh
Hi Matt, Check to make sure the A280 filter is clean.  Ours was filthy.  You might need to replace this (~$350).  We were experiencing the same thing you mentioned and just had the GE technician out today.  Also, make sure the A280 dial is set correctly on the lamp.  Please check the manual for