Yes, our new Akta prime has also only 2 mm path length and readings are, therefore, much smaller than with 1 cm path length in a typical spectro-photometer.
Best Marius > Hi all, > > We recently got low UV reading on our AKTA prime. We contacted GE > healthcare, but was told that the UV reading on AKTA prime should be > 20% of the spectrometer reading, because the path length of the flow > cell of AKTA prime is only 2 mm (20% of the pathlength of a > spectrometer cuvette). > > I am not sure whether that is the case, but we used to get much > higher UV readings on the same AKTA prime (almost the same as the > spectrometer reading). The UV reading just keeps dropping over time > in the past several months. > > Here I have two questions. First, should the UV reading on AKTA > prime be 20% of the spectrometer reading? Second, what could go wrong > with our AKTA prime? (I know it is not the lamp, because we put in a > new lamp and it didn't solve the problem) > > Thanks a lot! > Matt > > Dr.habil. Marius Schmidt Asst. Professor University of Wisconsin-Milwaukee Department of Physics Room 454 1900 E. Kenwood Blvd. Milwaukee, WI 53211 phone: +1-414-229-4338 email: m-schm...@uwm.edu http://users.physik.tu-muenchen.de/marius/
