Dear Savvas,
Since you mention the case of TolC explicitly, I should add to what
Pietro Roversi has already written in his message in relation to anisotropy
effects. Ben Luisi's group kindly let us have their data in the late 20th
century, so that we could use the then new and exciting log-li
Dear all,
one comment on the subject of white lines and anomalous phasing
power
- one has to be extremely careful to jump to conclusions
about the effect that a chemical treatment of the heavy atom (say
oxydation/reduction of Se) has
on the shape of the edge and the associated anomalous signa
I think that SeMet oxidation has been a problem in the past in at least one
case that I know, that of TolC by Koronakis et al. The same group addressed
these problems in more detail in a second paper (see below):
Crystal structure of the bacterial membrane protein TolC central to
multidrug eff
Hi,I believe it is not important as long as you run a proper scan of the
crystal. Both forms will allow proper phasing.
This is very well described in a paper from Thomazeau et al.
here is the reference
MAD on threonine synthase: the phasing power of oxidized selenomethionine.
Acta Crystallogr D B
Hi,
I wouldn't bother either. I once phased a structure based on about 160
oxidized Se in the AU (Hothorn et al., JBC, 2006). Just make sure that
most of them are oxidized and do a proper absorption scan.
best
Michael
aka akaka wrote:
Dear All
I would like to know whether oxidation of Se
Hi,
I wouldn't worry about Se oxidation. In principle having a mix of
oxidized/reduced seleniums is unfavorable, as you'll have less signal at the
edge (broadening). However, all-oxidized Se apparently makes things better
(sharper and more intense peak; I forgot the reference, i think it may
