Hi,I believe it is not important as long as you run a proper scan of the crystal. Both forms will allow proper phasing. This is very well described in a paper from Thomazeau et al. here is the reference
MAD on threonine synthase: the phasing power of oxidized selenomethionine. Acta Crystallogr D Biol Crystallogr.<javascript:AL_get(this,%20'jour',%20'Acta%20Crystallogr%20D%20Biol%20Crystallogr.');> 2001 Sep;57(Pt 9):1337-40. Epub 2001 Aug 23. Cheers, Pascal Egea, PhD Post Doctoral Researcher UCSF Department of Biochemistry and Biophysics Stroud laboratory On Mon, Feb 9, 2009 at 10:27 AM, aka akaka <druida...@hotmail.com> wrote: > Dear All > I would like to know whether oxidation of Se entails any problem for SAD or > MAD experiments and/ or how to resolve it. Cannot use DTT or reducing agents > in my protein (extracellular and disulphide bonds are important). > Thanks > > Dr. R.Depetris > Weill Cornell Medical College > > > > ------------------------------ > Get 5 GB of storage with Windows Live Hotmail. Sign up > today.<http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_5gb_112008> >
