I think that SeMet oxidation has been a problem in the past in at least one case that I know, that of TolC by Koronakis et al. The same group addressed these problems in more detail in a second paper (see below):
Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C. Nature. 2000 Jun 22;405(6789):914-9. Oxidation of selenomethionine: some MADness in the method! Sharff AJ, Koronakis E, Luisi B, Koronakis V. Acta Crystallogr D Biol Crystallogr. 2000 Jun;56(Pt 6):785-8. Best wishes Savvas ---- Savvas Savvides L-ProBE, Unit for Structural Biology Ghent University K.L. Ledeganckstraat 35 9000 Ghent, BELGIUM office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19 Email: savvas.savvi...@ugent.be http://www.lprobe.ugent.be/xray.html From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of aka akaka Sent: Monday, February 09, 2009 7:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Se oxidation Dear All I would like to know whether oxidation of Se entails any problem for SAD or MAD experiments and/ or how to resolve it. Cannot use DTT or reducing agents in my protein (extracellular and disulphide bonds are important). Thanks Dr. R.Depetris Weill Cornell Medical College _____ Get 5 GB of storage with Windows Live Hotmail. Sign up today. <http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_5gb_11 2008> E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11720 http://www.pctools.com/en/spyware-doctor-antivirus/
