Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

2019-09-24 Thread Sabine Buschmann
Dear Will, if the crystals grow in vapour-diffusion, you can try to overlay the reservoir with oil to slow down the crystallization process. I know at least two cases where this technique helped to get plate-shaped crystals into more 3D shape. http://scripts.iucr.org/cgi-bin/paper?S00218898960135

Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

2019-09-19 Thread Quyen Hoang
Hi Will, We had a similar case recently. Microseeding gave us single crystals (originally clusters), but still very thin plates as yours. We then mutated a couple surface lysine residues to alanine and that led to thicker crystals. The thin crystals were indexed as C2221, but the thicker ones rev

Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

2019-09-19 Thread Anastassis Perrakis
Microseeding for new conditions? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118815/ On Sep 19, 2019, at 17:30, William Richardson mailto:william.richard...@nottingham.ac.uk>> wrote: Dear colleagues, I would like some advice on crystal growth optimisation. I have been trying to crystallis