Re: [ccp4bb] How can improve diffraction quality

An excellent review of this subject was published not long ago: http://dx.doi.org/10.1107/S0907444905032130 It is even open access! But, in general the "trick" to improving diffraction is to get your molecules clean, get them to all adopt the same conformation, and then sit still. Adding a

Re: [ccp4bb] How can improve diffraction quality

Hello You can do dehydration of crystal to get better diffraction ...go through this reference Dehydration Converts DsbG Crystal Diffraction from Low to High Resolution Begoña Heras

Re: [ccp4bb] How can improve diffraction quality

Silver bullet Additive screens Methylation Construct boundaries in situ proteolysis Have you got a ligand? Stick in in there! Do you have an expression tag? Cleave it/leave it on. What is in your protein buffer? Mess about with that? Temperature gradients How is your purity? How quickly are you

Re: [ccp4bb] How can improve diffraction quality

Hi You don't give any details of how you are collecting your data or what the protein is (small, medium, large, solvent content, unit cell etc etc). But at first glance your crystal is huge by "modern day standards". Very often large crystals are of poorer overall quality than smaller ones.