Hi You don't give any details of how you are collecting your data or what the protein is (small, medium, large, solvent content, unit cell etc etc). But at first glance your crystal is huge by "modern day standards"..... Very often large crystals are of poorer overall quality than smaller ones.....
If you are trying to "freeze" a crystal of that size you are likely to run into problems with data quality. I would suggest trying a smaller crystal...... but without more information it is difficult to provide any real advice. Good luck! Liz Dr. Liz Duke Principal Beamline Scientist Diamond Light Source Harwell Science and Innovation Campus Chilton OX11 0DE UK Tel. 01235 778057 Mob. 07920 138148 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Afshan Begum Sent: 18 October 2011 12:45 To: ccp4bb Subject: [ccp4bb] How can improve diffraction quality Dear ccp4 user I am facing one crucial problem regarding diffraction. Actually the size of my crystal is good enough 0.5mm but it was diffracted only 4 A. The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and 25mM Na citrate. I really need your suggestions regarding how can i improve my diffraction quality? Your support is highly appreciable. Best Regards AFSHAN
