Hello
You can do dehydration of crystal to get better diffraction
.......go through this reference............
Dehydration Converts DsbG Crystal Diffraction from Low to High Resolution
Begoña
Heras<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DHeras,%2520Bego%25C3%25B1a%26authorID%3D6603651310%26md5%3Dd2d49a88c2751086c775153e3331f609&_acct=C000060364&_version=1&_userid=3424920&md5=6dbc863b94c3bf6340f12da6adee70a8>
1 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF1>
, Melissa A.
Edeling<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DEdeling,%2520Melissa%2520A.%26authorID%3D6603262033%26md5%3Dd57707bf83335a312588ce73d3bd730b&_acct=C000060364&_version=1&_userid=3424920&md5=4d0dd88d8b5e3162c388c8da007533b6>
1 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF1>
, Karl A.
Byriel<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DByriel,%2520Karl%2520A.%26authorID%3D6701558759%26md5%3D8cc9cd028d7292811a47d9ccd3e6d9a4&_acct=C000060364&_version=1&_userid=3424920&md5=95f75e9e035dbb96b2ac2458638a7acf>
1 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF1>
, Alun
Jones<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DJones,%2520Alun%26authorID%3D8156858700%26md5%3D992099f5dce064695b2b201c7ef62fa0&_acct=C000060364&_version=1&_userid=3424920&md5=5b36006773491de6f1fe3e11fd3f0362>
1 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF1>
, Satish
Raina<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DRaina,%2520Satish%26authorID%3D7005284671%26md5%3D339a4d07d0c405b35ede6cc65bd10b4a&_acct=C000060364&_version=1&_userid=3424920&md5=d179172315a3ce2212b520bd72cedf71>
2 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF2>
, Jennifer L.
Martin<http://www.sciencedirect.com/science?_ob=RedirectURL&_method=outwardLink&_partnerName=27983&_origin=article&_zone=art_page&_linkType=scopusAuthorDocuments&_targetURL=http%3A%2F%2Fwww.scopus.com%2Fscopus%2Finward%2Fauthor.url%3FpartnerID%3D10%26rel%3D3.0.0%26sortField%3Dcited%26sortOrder%3Dasc%26author%3DMartin,%2520Jennifer%2520L.%26authorID%3D8043271300%26md5%3Dbfbb451581631e88eb9aab78dba30ce2&_acct=C000060364&_version=1&_userid=3424920&md5=28050987b73ecd02a369b5486c0d6ce0>
1 <http://www.sciencedirect.com/science/article/pii/S0969212603000054#AFF1>
,
Structure, Vol. 11, 139–145, February, 2003,
On Tue, Oct 18, 2011 at 5:52 PM, Charles Allerston <
charles.allers...@sgc.ox.ac.uk> wrote:
> Silver bullet
> Additive screens
> Methylation
> Construct boundaries
> in situ proteolysis
> Have you got a ligand? Stick in in there!
> Do you have an expression tag? Cleave it/leave it on.
> What is in your protein buffer? Mess about with that?
> Temperature gradients
> How is your purity?
> How quickly are you getting this in to trays? Can you speed this up?
> Might be a factor.
> Annealing at the beamline?
>
> etc.
>
> cheers,
>
> charlie
>
>
> ________________________________________
> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Afshan
> Begum [afshan...@yahoo.com]
> Sent: 18 October 2011 12:45
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] How can improve diffraction quality
>
> Dear ccp4 user
>
> I am facing one crucial problem regarding diffraction. Actually the size of
> my crystal is good enough 0.5mm but it was diffracted only 4 A.
>
> The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and
> 25mM Na citrate. I really need your suggestions regarding how can i
> improve my diffraction quality?
>
> Your support is highly appreciable.
>
> Best Regards
>
> AFSHAN
>
--
Vandana kukshal
