.png@01D2B21A.8EA7C860]
Heisenberg was probably here!
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Kevin Jude
Sent: Monday, April 10, 2017 4:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] suggestion on protein crystallization optimization from
phase separation
I had
I had success once by varying the drop volume ratios as described here:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203341/
although we ended up getting data from a different crystal form.
~1 M LiSO4 is a surprising condition for cocrystallizing protein and DNA if
they aren't covalently or topol
Hope you get nice fat crystals soon!
Phoebe
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Johannes Cramer
[johannes.cra...@gmail.com]
Sent: Friday, April 07, 2017 7:37 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] suggestion on protein cryst
Dear Joseph,
You have probably already done so, but have you tried in- or decreasing your
protein+DNA concentration?
I had a similar problem with proteins that were concentrated too low. I had
to increase protein and decrease Ammonium sulfate (precipitant in my case)
concentration.
Cheers,
Johann
Dear all:
I would like to seek your suggestion on protein crystallization from
phase separation.
We recently observed many small round droplets shown in our
protein/DNA crystallization. Condition are 0.8M-1.6M LiSO4; 20mM
MgCl2; pH 5-8; protein conc. ~15mg/ml). The UV microscope confirms
those ar
