Dear Joseph, You have probably already done so, but have you tried in- or decreasing your protein+DNA concentration? I had a similar problem with proteins that were concentrated too low. I had to increase protein and decrease Ammonium sulfate (precipitant in my case) concentration.
Cheers, Johannes 2017-04-06 17:16 GMT+02:00 Joseph Ho <sbddintai...@gmail.com>: > Dear all: > > I would like to seek your suggestion on protein crystallization from > phase separation. > We recently observed many small round droplets shown in our > protein/DNA crystallization. Condition are 0.8M-1.6M LiSO4; 20mM > MgCl2; pH 5-8; protein conc. ~15mg/ml). The UV microscope confirms > those are protein-rich phase separation. > We have tried to change conc. of LiSO4 and pH. Still we got different > size and amount of small round droplets. At 20 degree, those droplets > appear within one day and at 4 degree, it takes two-three days. We > also tried additive and silver bullet screen. So far, we have not > found a condition to have protein crystals. The protein is already > truncated. Several DNA constructs are on-going. > At this point, I would like to seek your advice on the method to > optimize the condition. Based on > > > PS. Any people have luck with protein crystallization by streaking the > Gelationous protein to new drop as shown in > http://xray.bmc.uu.se/terese/tutorial3.html . Can you please share > your experience with us? > > Thanks for your help. > > Joseph Ho >
