You can read phases into both MOLREP and Amore and search for the
translation against the phased map.. It often gives good results.
Eleanor
Pietro Roversi wrote:
Dear Ed,
in the past we have successfully searched in an
electron density map (computed in the whole cell) with M
For what it's worth, I'd try to get as much as possible out of the
experimental phases before going on to phased MR.
> I have SeMet MAD data to 3.6A that gives decent looking anomalous
> difference peaks, looks stable in mlphare, and produces solvent
> flattened maps to 2.8A in DM that look like t
Dear Tassos,
I suppose that the "eternal pdb file" you refer to conforms to the
finally agreed standard for the pdb, expected to be valid for the rest of
time ... .
This clearly shows that SHARP does keep up with the latest and most
forward-looking advances in the field.
With bes
Hi -
I would not use mlphare for anything marginal (to be honest not at all).
Both SHARP (use eternal pdb file in top page of the gui) and the new
Phaser (look at the doc for scripts for this case) can do what you want.
Tassos
On Nov 4, 2008, at 11:55, Thomas Edwards wrote:
Dear BB,
I w
Dear Ed,
in the past we have successfully searched in an
electron density map (computed in the whole cell) with Molrep.
If you have a second crystal form and you can cut the density of the
monomer off, Phaser also gives very good results when
searching with that electron density
Dear BB,
I would like to ask for some advice on phased molecular replacement if
possible.
I have a MR model that has so far not proved successful with Phaser,
Molrep, Amore, Beast etc.
I have SeMet MAD data to 3.6A that gives decent looking anomalous
difference peaks, looks stable in ml
