Dear Tassos,
I suppose that the "eternal pdb file" you refer to conforms to the
finally agreed standard for the pdb, expected to be valid for the rest of
time ... .
This clearly shows that SHARP does keep up with the latest and most
forward-looking advances in the field.
With best wishes,
Gerard.
--
On Tue, Nov 04, 2008 at 01:38:25PM +0100, Anastassis Perrakis wrote:
> Hi -
>
> I would not use mlphare for anything marginal (to be honest not at all).
>
> Both SHARP (use eternal pdb file in top page of the gui) and the new Phaser
> (look at the doc for scripts for this case) can do what you want.
>
> Tassos
>
>
> On Nov 4, 2008, at 11:55, Thomas Edwards wrote:
>
>> Dear BB,
>>
>> I would like to ask for some advice on phased molecular replacement if
>> possible.
>>
>> I have a MR model that has so far not proved successful with Phaser,
>> Molrep, Amore, Beast etc.
>>
>> I have SeMet MAD data to 3.6A that gives decent looking anomalous
>> difference peaks, looks stable in mlphare, and produces solvent flattened
>> maps to 2.8A in DM that look like the density might be a sensible shape -
>> wrt solvent gaps etc - but not interpretable so far (I will be trying
>> phasing in SHARP, CNS etc).
>> In the mean time, is there a good way to combine phases that may be
>> slightly sensible with molecular replacement? Things may also be
>> complicated by the possibility of NCS translations…
>>
>> Any advice gratefully received.
>>
>> Many thanks to all those who continue to provide excellent suggestions,
>> Cheers
>> Ed
>>
>> ______________
>> T.Edwards Ph.D.
>> Garstang 8.53d
>> Astbury Centre for Structural Molecular Biology
>> University of Leeds, Leeds, LS2 9JT
>> Telephone: 0113 343 3031
>> http://www.bmb.leeds.ac.uk/staff/tae/
>> If you're not part of the solution, you're part of the precipitate. ~Henry
>> J. Tillman
>>
>>
>
--
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