Dear BB,
I would like to ask for some advice on phased molecular replacement if possible. I have a MR model that has so far not proved successful with Phaser, Molrep, Amore, Beast etc. I have SeMet MAD data to 3.6A that gives decent looking anomalous difference peaks, looks stable in mlphare, and produces solvent flattened maps to 2.8A in DM that look like the density might be a sensible shape - wrt solvent gaps etc - but not interpretable so far (I will be trying phasing in SHARP, CNS etc). In the mean time, is there a good way to combine phases that may be slightly sensible with molecular replacement? Things may also be complicated by the possibility of NCS translations... Any advice gratefully received. Many thanks to all those who continue to provide excellent suggestions, Cheers Ed ______________ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 <http://www.bmb.leeds.ac.uk/staff/tae/> <http://www.bmb.leeds.ac.uk/staff/tae/Research> http://www.bmb.leeds.ac.uk/staff/tae/ If you're not part of the solution, you're part of the precipitate. ~Henry J. Tillman
