Dear Harvey,
I had very good results with low melt agarose and the detergent screen
(e.g. Hampton Research). In both cases, the tendency for cluster formation
as well as the diffraction power could be improved.
Anja
> Dear CCP4BBers,
>
> Recently I got a crystal which appeared in the condition
: [ccp4bb] optimization of dumbbell like crystal
Dear CCP4BBers,
Recently I got a crystal which appeared in the condition 2.1M DL-Malic
Acid, pH 7.0. The crystal looks like a dumbbell but was composed of a
cluster of very thin needles as shown in the picture. The crystal can be
repeated but the
Additives screening.
YB
2011-02-21
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发件人： Bosch, Juergen
发送时间： 2011-02-21 03:46:12
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] optimization of dumbbell like crystal
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at 9
Dear harvey
Consider your purification and preparation protocols - is the sample pure and
homogenous?
Poul
On 21/02/2011, at 03.29, Harvey Rodriguez wrote:
> Dear CCP4BBers,
>
> Recently I got a crystal which appeared in the condition 2.1M DL-Malic Acid,
> pH 7.0. The crystal looks like a d
Dear Harvey
Based on the experimental history you provided I would try two quick things in
order to obtain additional dimensions for your condition.
(1) set up your best consensus condition against an additive screen (e.g. From
Hampton).
(2) I would repeat your last pH vs precipitant grid agai
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at 9:29 PM, Harvey Rodriguez wrote:
Dear CCP4BBers,
Recently I got a crystal which appeared in the condition 2.1M DL-Malic Acid, pH
7.0. The crystal looks like a dumbbell but was comp
