Additives screening. YB
2011-02-21 yybbll 发件人： Bosch, Juergen 发送时间： 2011-02-21 03:46:12 收件人： CCP4BB 抄送： 主题： Re: [ccp4bb] optimization of dumbbell like crystal Seeding ? changing from sitting to hanging drop or under oil ? play with temperature ? Jürgen On Feb 20, 2011, at 9:29 PM, Harvey Rodriguez wrote: Dear CCP4BBers, Recently I got a crystal which appeared in the condition 2.1M DL-Malic Acid, pH 7.0. The crystal looks like a dumbbell but was composed of a cluster of very thin needles as shown in the picture. The crystal can be repeated but the diffraction is poor. I have tried the grid optimization by pH and salt concentration gradients, however there was no improvement in either the shape or the quality of the crystal. Does anyone have some experiences in optimizing this kind of crystals? Any suggestion is appreciated! Harvey <dumbbell crystals.jpg> ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
