Sorry for the slew of offtopic posts, but does anyone here have any
experience repacking the large 120mL Superdex75/200 columns? Any
advice/tips on doing it? I've got an older column that's gotten clogged
while washing w/NaOH (can't go over 0.1mL/min w/o getting overpressure
alarm), and not sur
checked the theoretical plate
> number of our 26/60 after repacking, it was >11000, not as high as the
> 13000 stated in the 26/60 new column manual, but it was good enough for us.
>
> Zhijie
>
> ------**------------
> From: "Peter Hsu
Zhijie
--
From: "Peter Hsu"
Sent: Thursday, July 12, 2012 9:51 PM
To:
Subject: [ccp4bb] offtopic: packing gel filtration columns
Hi all,
Sorry for the slew of offtopic posts, but does anyone here have any
experience repacking the large 120mL Superdex75/200 co
> Subject: Re: [ccp4bb] offtopic: packing gel filtration columns
>
> We do it pretty routinely in our lab with great results. To do it right you
> have to invest in a reservoir sold by GE. It screws onto the end of the
> column. It allows you to pour the entire slury (resin and
> From: Yarrow Madrona
> Date: July 12, 2012 7:39:57 PM PDT
> To: Peter Hsu
> Subject: Re: [ccp4bb] offtopic: packing gel filtration columns
>
> It is also likely that the clogging due to NAOH is due to crapped out
> protein. Try cleaning with guanadine.
>
>
&g
Hi Peter,
Most of the time it is just a clogged top filter. While the column is
running, loosen the top o-ring by loosening the black knob on the top.
Once that is done, carefully unscrew the white threaded adapter several
turns to back the top filter off the resin bed. You can then unscrew the
Hi all,
Sorry for the slew of offtopic posts, but does anyone here have any experience
repacking the large 120mL Superdex75/200 columns? Any advice/tips on doing it?
I've got an older column that's gotten clogged while washing w/NaOH (can't go
over 0.1mL/min w/o getting overpressure alarm), and
