> From: Yarrow Madrona <[email protected]>
> Date: July 12, 2012 7:39:57 PM PDT
> To: Peter Hsu <[email protected]>
> Subject: Re: [ccp4bb] offtopic: packing gel filtration columns
>
> It is also likely that the clogging due to NAOH is due to crapped out
> protein. Try cleaning with guanadine.
>
>
>
> On Jul 12, 2012, at 6:51 PM, Peter Hsu <[email protected]> wrote:
>
>> Hi all,
>>
>> Sorry for the slew of offtopic posts, but does anyone here have any
>> experience repacking the large 120mL Superdex75/200 columns? Any advice/tips
>> on doing it? I've got an older column that's gotten clogged while washing
>> w/NaOH (can't go over 0.1mL/min w/o getting overpressure alarm), and not
>> sure the bossman would be thrilled w/buying another column.
>>
>> Thanks for any ideas.
>>
>> Peter
>>
