> Subject: Re: [ccp4bb] offtopic: packing gel filtration columns > > We do it pretty routinely in our lab with great results. To do it right you > have to invest in a reservoir sold by GE. It screws onto the end of the > column. It allows you to pour the entire slury (resin and water) into the > resin in one shot. The reservoir is capped with an adapter and the pump > turned onto 10ml/min or so (less than the hardware pressure limit of 0.5 > mPascal). > > When the resin is packed you take the water and reservoir off. Then stick in > the plunger and continue to pack. when pushing down the plunger you have to > loosen the fitting that connects the akta tubing to the plunger tubing, so > that the excess liquid has somewhere to go. When you tighten the plunger to > hold it in place you will need to screw the fitting in ASAP. > > It is much easier to see than explain. Be careful of over tightening the > plunger as this will actually unscrew a fitting inside the plunger and the > column will leak. > > GE had a tutorial video on their website at one time. I don't know if it is > still up. It's one of those things that has to be done or seen rather then > explained. > > Good luck. > > > > > On Jul 12, 2012, at 6:51 PM, Peter Hsu <hsuu...@u.washington.edu> wrote: > >> Hi all, >> >> Sorry for the slew of offtopic posts, but does anyone here have any >> experience repacking the large 120mL Superdex75/200 columns? Any advice/tips >> on doing it? I've got an older column that's gotten clogged while washing >> w/NaOH (can't go over 0.1mL/min w/o getting overpressure alarm), and not >> sure the bossman would be thrilled w/buying another column. >> >> Thanks for any ideas. >> >> Peter >>
