See the following publication for an interesting method to resolve
pseudo-merohedral twinning in SeMet crystals:
Cansizoglu, A. E. and Chook, Y. M. (2007) Conformational heterogeneity
of Karyopherinβ2 is segmental. Structure, 15(11):1431-1441.
"In an effort to obtain single selenomethionine
Yes, on both counts. SeMet proteins can be frustratingly* close to
their native forms - with little quirks like twinning or lack of
useful diffraction etc. It's common practice to micro (or macro) seed
with native crystals and it often works quite well. It's fun and
sometimes useful to add a few ul
Dear All,
My SeMet protein crystals have a needle-like morphology, worse than that of
the native xtals. Also, although the cell dimensions of both forms is very
much similar, the SeMet xtals are twinned(as indicated by Ctruncate plots).
I was wondering if such cases were commonly seen, also is it
