See the following publication for an interesting method to resolve
pseudo-merohedral twinning in SeMet crystals:
Cansizoglu, A. E. and Chook, Y. M. (2007) Conformational heterogeneity
of Karyopherinβ2 is segmental. Structure, 15(11):1431-1441.
"In an effort to obtain single selenomethionine Kapβ2 crystals,
mixtures of selenomethionine and native proteins were crystallized. A
1:1 molar mixture of the two proteins gave single crystals..."
Diana
On Sep 1, 2010, at 10:37 PM, amit sharma wrote:
Dear All,
My SeMet protein crystals have a needle-like morphology, worse than
that of the native xtals. Also, although the cell dimensions of both
forms is very much similar, the SeMet xtals are twinned(as indicated
by Ctruncate plots). I was wondering if such cases were commonly
seen, also is it alright to microseed my SeMet protein drops with
seeds from the native xtal. I would be grateful if someone could
shed light on this.
thanks in advance,
--
Amit Sharma,
Postdoctoral Fellow,
Department of Biophysics,
Johns Hopkins University,
Baltimore,
MD21218
* * * * * * * * * * * * * * * * * * * * * * * * * * * *
Diana R. Tomchick
Associate Professor
University of Texas Southwestern Medical Center
Department of Biochemistry
5323 Harry Hines Blvd.
Rm. ND10.214B
Dallas, TX 75390-8816, U.S.A.
Email: diana.tomch...@utsouthwestern.edu
214-645-6383 (phone)
214-645-6353 (fax)
