Dear Christian,
Kleywegt (1996; "Use of Non-crystallographic Symmetry in Protein
Structure Refinement." Acta Cryst D52, 842-857. DOI:
10.1107/S0907444995016477) reported that NCS-related molecules tend to
be similar but differences in ligand binding do sometimes occur (cited
Sevcik et al. 1996 in
Dear all,
the tenor seems to be, if you see the ligand in any of the
monomers, that's the mode the ligand binds. We have seen other cases,
though, too.
Usually the protein I am thinking of (TetR(D)) crystallizes with one
molecules /a.u. In solution it is an obligate dimer. The dimer is
forme
Hi Matthias,
Thank you for response. Could you provide one or more pdb codes as example?
Best,
Christian
*From:* Barone, Matthias
*Subject:* [ccp4bb] ligand bound to only one chain in the crystal
*Date:* Tuesday, October
Hi Christian
I wouldn't worry: if the density is clear that nails it. You didn't say
whether this is a soak or co-crystallization. I assume the former since
you mention solvent channels. In my experience this is much more likely in
the case of soaks, which can often (though not always) be ratio
, 2020 11:19:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ligand bound to only one chain in the crystal
Hello,
In our structure only one chain in a crystallographic trimer (non-biological)
shows a ligand bound to it (with clear density). There doesn't seem to be any
channels (or lack of
] ligand bound to only one chain in the crystal
Hello,
In our structure only one chain in a crystallographic trimer (non-biological)
shows a ligand bound to it (with clear density). There doesn't seem to be any
channels (or lack of them) favoring that specific site. Can the community give
==
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Christian
GALICIA [christian.galicia.diaz.sant...@vub.be]
Sent: 27 October 2020 11:19
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ligand bound to only one chain in the crystal
Hello,
In our structure only one chain in a
Hello,
In our structure only one chain in a crystallographic trimer
(non-biological) shows a ligand bound to it (with clear density). There
doesn't seem to be any channels (or lack of them) favoring that specific
site. Can the community give your opinion on whether this can make the
presence o
